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Determination of Antagonistic Activities of Endophytic Bacteria Isolated from Different Wheat Genotypes Against Fusarium culmorum

Yıl 2024, Cilt: 10 Sayı: 1, 96 - 116, 29.04.2024

İnci Güler Güney Sibel Derviş Göksel Özer Hüsnü Aktaş Mehmet Ata Keske

https://doi.org/10.24180/ijaws.1386741

Öz

This study aimed to evaluate the physiological and biochemical properties and enzyme activities of endophytic bacteria obtained from different wheat genotypes, as well as their effectiveness against Fusarium culmorum, which causes root and crown rot in wheat. The results obtained from double culture tests of isolates against F. culmorum showed that the inhibition rate varied between 80.56% and 13.90%. The inhibition rate against F. culmorum was 80.59% for Bacillus subtilis (MM11), 69.41% for Stenotrophomonas maltophilia (EY5), and 61.10% for Enterobacter sp. (MY3) under in vitro conditions, the most effective isolates. Pseudomonas putida (EM9) and Pseudomonas orientalis (MM21) isolates gave positive results in all tests in the production of amylase, cellulase, phosphatase, ACC deaminase, and siderophore. To identify six promising isolates, 16S rRNA gene-based sequence analysis was utilized. The efficacy of bacterial strains against F. culmorum, pot experiments were conducted in a growth room (in vivo). The results demonstrated that the combination of S. maltophilia, Enterobacter sp., and B. subtilis (MY3+EY5+MM11) yielded the most favorable outcomes in terms of disease severity, plant height, wet weight, dry weight, root wet weight, and root dry weight. The combination of Stenotrophomonas rhizophila, P. putida, and P. orientalis (EY1+EM9+MM21) exhibited promising results. Utilizing effective bacterial strains is anticipated to reduce the dependence on and costs associated with chemical fertilizers and pesticides while minimizing their environmental impact. Furthermore, these strains show potential for commercial applications pending further validation procedures. The findings from this study significantly contribute to the field of biological control strategies against F. culmorum by leveraging the diverse capabilities of endophytic bacteria.

Anahtar Kelimeler

Endophyte Fusarium culmorum biological control wheat

Destekleyen Kurum

MARDIN ARTUKLU UNİVERSİTY

Proje Numarası

MAÜ.BAP.20.KMY.018

Teşekkür

I acknowledge the support and funding provided by Mardin Artuklu University for Project Number MAÜ.BAP.20.KMY.018

Kaynakça

  • Akinsanmi, O. A., Mitter, V., Simpfendorfer, S., Backhouse, D., & Chakraborty, S. (2004). Identity and pathogenicity of Fusarium spp. isolated from wheat fields in Queensland and northern New South Wales. Australian Journal of Agricultural Research, 55, 97–107. https://doi.org/10.1071/AR03090
  • Aktaş, H., Tunalı, B., Bostancıoğlu, H., & Bayram, E. (1997). Reaction of some wheat varities and lines against to root and foot rot disease agents in the field and laboratory conditions. Journal of Turkish Phytopathology, 26, 61–68.
  • Alkan, M. , Göre, M. E., Bayraktar, H., & Özer, G. (2019). Kışlık buğdaylarda kök ve kökboğazi çürüklüğüne sebep olan Fusarium spp. izolatlari arasindaki genetik varyasyonun retrotranzpozon temelli iPBS markörleri ile incelenmesi. Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi, 5(2), 250–259. https://doi.org/10.24180/ijaws.537423
  • Alkan, M., Özer, G., İmren, M., Özdemir, F., Morgounov, A., & Dababat, A. A. (2021). First report of Fusarium culmorum and Microdochium bolleyi causing root rot on triticale in Kazakhstan. Plant Disease, 105(7), 2015. https://doi.org/10.1094/PDIS-12-20-2659-PDN
  • Arıcı, Ş. E., Arap, Ü., & Yatağan, F. B. (2013). Isparta ve Burdur illeri buğday ekim alanlarındaki kök ve kök boğazı fungal hastalık etmenlerinin belirlenmesi. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 17(2), 26–30.
  • Baker, G. C., Smith, J. J., & Cowan, D. A. (2003). Review and re-analysis of domain-specific 16S primers. Journal of Microbiological Methods, 55(3), 541–555. https://doi:10.1016/j.mimet.2003.08.009
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  • Brader, G., Compant, S., Mitter, B., Trognitz, F., & Sessitsch, A. (2014). Metabolic potential of endophytic bacteria. Current Opinion in Biotechnology, 27, 30–7. https://doi.org/10.1016/j.copbio.2013.09.012
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  • Chernin. L., & Chet, I. (2002). Microbial enzymes in the biocontrol of plant pathogens and pests. In: Burns, R. G.; Dick, R. P. ed. Enzymes in the environment: activity, ecology and applications. New York, Marcel Dekker. Pp 171-226.
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Farklı Buğday Genotiplerinden İzole Edilen Endofitik Bakterilerin Fusarium culmorum'a Karşı Antagonistik Aktivitelerinin Belirlenmesi

Yıl 2024, Cilt: 10 Sayı: 1, 96 - 116, 29.04.2024

İnci Güler Güney Sibel Derviş Göksel Özer Hüsnü Aktaş Mehmet Ata Keske

https://doi.org/10.24180/ijaws.1386741

Öz

Bu araştırmada, farklı buğday genotiplerinden elde edilen endofitik bakterilerin, fizyolojik ve biyokimyasal özelliklerinin ile enzim aktivitelerinin yanısıra buğdayda kök ve kökboğazı çürüklüğüne neden olan Fusarium culmorum’a karşı etkinliğinin değerlendirilmesi amaçlanmıştır. İzolatların F. culmorum’a karşı ikili kültür testlerinden elde edilen sonuçlara göre inhibisyon oranı %80.56- %13.90 arasında değişiklik göstermiştir. In vitro koşullarda Fusarium culmorum’a karşı inhibisyon oranı %80.59 oranıyla Bacillus subtilis (MM11), %69.41 oranıyla Stenotrophomonas maltophilia (EY5) ve %61.10 oranıyla Enterobacter sp. (MY3) en etkili izolatlar olmuştur. Amilaz, selülaz, fosfataz, ACC deaminaz ve siderofor üretiminde Pseudomonas putida (EM9) ve Pseudomonas orientalis (MM21) izolatları tüm testte pozitif sonuç vermiştir. Umut vadeden altı izolat 16S rRNA geninin sekans analizi kullanılarak tanımlanmıştır. Bakteri suşlarının F. culmorum’a karşı etkinliğini değerlendirmek için iklim odası koşullarında (in vivo) saksı denemeleri kurulmuştur. Sonuçlar, Enterobacter sp., S. maltophilia ve B. subtilis (MY3+EY5+MM11) kombinasyonunun hastalık şiddeti, bitki boyu, yaş ağırlık, kuru ağırlık, kök yaş ağırlığı ve kök kuru ağırlığı açısından en uygun sonuçları verdiğini göstermiştir. Stenotrophomonas rhizophila, P. putida ve P. orientalis (EY1+EM9+MM21) kombinasyonu da oldukça etkili olmuştur. Etkili bakteri suşlarının kullanılmasının, çevresel etkilerini en aza indirirken, kimyasal gübre ve pestisitlere olan bağımlılığı ve bunlarla ilişkili maliyetleri azaltması beklenmektedir. Ek doğrulama prosedürlerinin ardından bu suşların, ticari uygulamalar için potansiyelinin olduğu düşünülmektedir. Bulgular, F. culmorum’a karşı çeşitli endofitik bakterilerin kullanıldığı biyolojik kontrol çalışmalarına katkıda bulunacaktır.

Anahtar Kelimeler

Endofit Fusarium culmorum biyolojik mücadele buğday

Destekleyen Kurum

Mardin Artuklu University

Proje Numarası

MAÜ.BAP.20.KMY.018

Teşekkür

I acknowledge the support and funding provided by Mardin Artuklu University for Project Number MAÜ.BAP.20.KMY.018

Kaynakça

  • Akinsanmi, O. A., Mitter, V., Simpfendorfer, S., Backhouse, D., & Chakraborty, S. (2004). Identity and pathogenicity of Fusarium spp. isolated from wheat fields in Queensland and northern New South Wales. Australian Journal of Agricultural Research, 55, 97–107. https://doi.org/10.1071/AR03090
  • Aktaş, H., Tunalı, B., Bostancıoğlu, H., & Bayram, E. (1997). Reaction of some wheat varities and lines against to root and foot rot disease agents in the field and laboratory conditions. Journal of Turkish Phytopathology, 26, 61–68.
  • Alkan, M. , Göre, M. E., Bayraktar, H., & Özer, G. (2019). Kışlık buğdaylarda kök ve kökboğazi çürüklüğüne sebep olan Fusarium spp. izolatlari arasindaki genetik varyasyonun retrotranzpozon temelli iPBS markörleri ile incelenmesi. Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi, 5(2), 250–259. https://doi.org/10.24180/ijaws.537423
  • Alkan, M., Özer, G., İmren, M., Özdemir, F., Morgounov, A., & Dababat, A. A. (2021). First report of Fusarium culmorum and Microdochium bolleyi causing root rot on triticale in Kazakhstan. Plant Disease, 105(7), 2015. https://doi.org/10.1094/PDIS-12-20-2659-PDN
  • Arıcı, Ş. E., Arap, Ü., & Yatağan, F. B. (2013). Isparta ve Burdur illeri buğday ekim alanlarındaki kök ve kök boğazı fungal hastalık etmenlerinin belirlenmesi. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 17(2), 26–30.
  • Baker, G. C., Smith, J. J., & Cowan, D. A. (2003). Review and re-analysis of domain-specific 16S primers. Journal of Microbiological Methods, 55(3), 541–555. https://doi:10.1016/j.mimet.2003.08.009
  • Berg, G., & Hallmann, J. (2006). Control of plant pathogenic fungi with bacterial endophytes. pp. 53–69. Springer-Verlag.
  • Bozoğlu, T., Derviş, S., Imren, M., Amer, M., Özdemir, F., Paulitz, T.C., Morgounov, A., Dababat, A.A., & Özer, G. (2022). Fungal pathogens associated with crown and root rot of wheat in Central, Eastern, and Southeastern Kazakhstan. Journal of Fungi, 8(5), 417. https://doi.org/10.3390/jof8050417
  • Brader, G., Compant, S., Mitter, B., Trognitz, F., & Sessitsch, A. (2014). Metabolic potential of endophytic bacteria. Current Opinion in Biotechnology, 27, 30–7. https://doi.org/10.1016/j.copbio.2013.09.012
  • Cappuccino, J. C., & Sherman, N. (1992). In Microbiology: A Laboratory Manual Third Ed. Benjamin/ Cummings Pub. Co., New York, pp. 125–179
  • Chen, C., Bauske, E. M., Musson, G., Rodrġguezkabana, R., & Kloepper, J. W. (1995). Biological control of Fusarium wilt on cotton by use of endophytic bacteria. Biological Control, 5, 83–91. https://doi.org/10.1006/bcon.1995.1009
  • Chernin. L., & Chet, I. (2002). Microbial enzymes in the biocontrol of plant pathogens and pests. In: Burns, R. G.; Dick, R. P. ed. Enzymes in the environment: activity, ecology and applications. New York, Marcel Dekker. Pp 171-226.
  • Cook, R. J. (2001) Management of wheat and barley root diseases in modern farming systems. Australasian Plant Pathology, 30, 119–126. https://doi.org/10.1071/AP01010
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  • Gökçe, A.Y., & Kotan, R. (2016). Buğday kök çürüklüğüne neden olan Bipolaris sorokiniana (Sacc.)’ya karşı PGPR ve biyoajan bakterileri kullanılarak kontrollü koşullarda biyolojik mücadele imkanlarının araştırılması. Bitki Koruma Bülteni, 56, 49–75.
  • Holt, G. J., Krieg, N. R., Sneath, P. H., Staley, J. T., & Williams, S. T. (1994). In: Bergey's Manual of Determinative Bacteriology. ninth ed. The Williams and Wilkins Pub., M. D., USA. Growth Applied and Environmental Microbiology, 66, 948–955.
  • Jankiewicz, U., Brzezinska, M. S., & Saks, E. (2012). Identification and characterization of a chitinase of Stenotrophomonas maltophilia, a bacterium that is antagonistic towards fungal phytopathogens. Journal of Bioscience and Bioengineering, 113(1), 30–35. https://doi.org/10.1016/j.jbiosc.2011.08.023
  • Jasim, B., Jimtha, John C., Mathew, J., & Radhakrishnan, E. K. (2013). Plant growth promoting potential of endophytic bacteria isolated from Piper nigrum. Plant Growth Regulation, 71, 1–11. https://doi.org/10.1007/s10725-013-9802-y
  • Ji, P., & Wilson, M. (2002). Assessment of the importance of similarity in carbon source utilization profiles between the biological control agent and the pathogen in bilogical control of bacterial speck of tomato. Applied and Environmental Microbiology, 68, 4383–4389. https://doi.org/10.1128/AEM.68.9.4383-4389.2002
  • Khan, A. L., Hussain, J., Al-Harrasi, A., Al-Rawahi, A., & Lee, I. J. (2015). Endophytic fungi: resource for gibberellins and crop abiotic stress resistance. Critical Reviews in Biotechnology, 35, 62–74. https://doi.org/10.3109/07388551.2013.800018
  • Kloepper, J. W., Tuzun, S., & Kuc, J. A. (1992). Proposed definitions related to induced disease resistance. Biocontrol Science and Technology, 2, 349–351. https://doi.org/10.1080/09583159209355251
  • Kosiak, B., Skjerve, E., Thrane, U., & Torp, M. (2003). The prevalence and distribution of Fusarium species in Norwegian cereals: a survey. Acta Agriculturae Scandinavica 53:168–176. https://doi.org/10.1080/09064710310018118
  • Lodewyckx, C., Vangronsveld, J., Porteous, F., Moore, E. R. B., Taghavi, S., Mezgeay, M., & Van Der Lelie, D. (2002). Endophytic bacteria and their potential applications. Critical Reviews in Plant Sciences, 21, 583–606. https://doi.org/10.1080/0735-260291044377
  • Mnasri, N., Chennaoui, C., Gargouri, S., Mhamdi, R., Hessini, K., Elkahoui, S., & Djébali, N. (2017). Efficacy of some rhizospheric and endophytic bacteria in vitro and as seed coating for the control of Fusarium culmorum infecting durum wheat in Tunisia. European Journal of Plant Pathology, 147, 501–515. https://doi.org/10.1007/s10658-016-1018-3
  • Nautiyal, C. S. (1999). An efficient microbiological growth medium forscreening phosphate solubilizing microorganisms. FEMS MicrobiologyLetters, 170(1), 265–270. https://doi.org/10.1111/j.1574-6968.1999.tb13383.x
  • Nourozian, J., Etebarian, H.R., & Khodakaramian, G. (2006). Biological control of Fusarium graminearum on wheat by antagonistic bacteria. Songklanakarin Journal of Science and Technology, 28(Suppl. 1), 29–38.
  • Owen, N. L., & Hundley, N. (2004). Endophytes-the chemical synthesizers inside plants. Science Progress, 87, 79–99. https://doi.org/10.3184/003685004783238553
  • Özer, G., Paulitz, T. C., Imren, M., Alkan, M., Muminjanov, H., & Dababat, A. A. (2020). Identity and pathogenicity of fungi associated with crown and root rot of dryland winter wheat in Azerbaijan. Plant Disease, 104(8), 2149–2157. https://doi.org/10.1094/PDIS-08-19-1799-RE
  • Özer, G., Erper, İ., Yıldız, Ş., Bozoğlu, T., Zholdoshbekova, S., Alkan, M., Tekin, F., Uulu, T. E., İmren, M., Dababat, A. A., & Derviş, S. (2023). Fungal pathogens associated with crown and root rot in wheat-growing areas of Northern Kyrgyzstan. Journal of Fungi, 9, 124. https://doi.org/10.3390/jof9010124
  • Pang, F.,Tao, A., Ayra-Pardo, C., Wang, T.,Yu, Z., & Huang, S. (2022). Plant organ- and growth stage-diversity of endophytic bacteria with potential as biofertilizers isolated from wheat (Triticum aestivum L.). BMC Plant Biology, 22, 276. https://doi.org/10.1186/s12870-022-03615-8
  • Pleban, S., Ingel, F., & Chet, I. (1995). Control of Rhizoctonia solani and Sclerotium rolfsii by use of endophytic bacteria (Bacillus spp.). European Journal of Plant Pathology, 101: 665–672. https://doi.org/10.1007/BF01874870
  • Quartana, C., Faddetta, T., Anello, L., Bernardo, M.,Petralia, R., & Campanella, V. (2022). Activity of bacterial seed endophytes of landrace durum wheat for control of Fusarium foot rot. Phytopathologia Mediterranea 61(1), 95–106. https://doi.org/10.36253/phyto-12993
  • Reinhold-Hurek, B., & Hurek, T. (2011). Living inside plants: bacterial endophytes. Current Opinion in Plant Biology, 14, 435–43. https://doi.org/10.1016/j.pbi.2011.04.004
  • Ryan, R. P., Germaine, K., Franks, A., Ryan, D. J., & Dowling, D. N. (2008). Bacterial endophytes: recent developments and applications. FEMS Microbiol Letters, 278, 1–9. https://doi.org/10.1111/j.1574-6968.2007.00918.x
  • Schwyn, B., & Neilands, J. B. (1987). Universal chemical assay for the detection and determination of siderophores. Analytical Biochemistry, 160, 47–56. https://doi.org/10.1016/0003-2697(87)90612-9
  • Shikur Gebremariam, E., Sharma-Poudyal, D., & Paulitz, T. C. (2018). Identity and pathogenicity of Fusarium species associated with crown rot on wheat (Triticum spp.) in Turkey. European Journal of Plant Pathology, 150, 387–399. https://doi.org/10.1007/s10658-017-1285-7
  • Shi, Y., Lou, K., & Li, C. (2009). Promotion of plant growth by phytohormone-producing endophytic microbes of sugar beet. Biology and Fertility of Soils, 45, 645–653. https://doi.org/10.1007/s00374-009-0376-9
  • Singh, P. P., Shin, Y. C., Park, C. S., & Chung, Y. R. (1999). Biological control of Fusarium wilt of cucumber by chitinolytic bacteria. Phytopathology, 89, 92–99. https://doi.org/10.1094/PHYTO.1999.89.1.92
  • Soylu, S. , Sülü, S. M., & Bozkurt, İ. A. (2016). Bitki büyüme düzenleyici ve biyolojik mücadele etmeni olarak bakteriyel endofitler. Mustafa Kemal Üniversitesi Ziraat Fakültesi Dergisi, 21(1), 103–111. https://dergipark.org.tr/tr/pub/mkuzfd/issue/24548/260030
  • TURKSTAT (2023). Turkish Statistical Institute, Agricultural Istatistics, Ankara. https://biruni.tuik.gov.tr/medas/?kn=92&locale=tr. [Access date: 27.04.2023].
  • Tunalı, B., Nicol, J. M., Hadson, D., Uçkun, Z., Büyük, Z. O., Erdurmuş, D., Hekimhan, H., Aktaş, M.A., Akbudak, M.A., & Bağcı, S. A. (2008). Root and crown rot fungi associated with spring, facultative, and winter wheat in Turkey. Plant Disease, 92:1299–1306. https://doi.org/10.1094/PDIS-92-9-1299
  • Türkölmez, Ş., Özer, G., & Derviş, S. (2023). Clonostachys rosea Strain ST1140: An endophytic plant-growth-promoting fungus, and its potential use in seedbeds with wheat-grain substrate. Current Microbiology, 80(1), 36. https://doi.org/10.1007/s00284-022-03146-3
  • Wagacha, J. M., & Muthomi, J. W. (2007). Fusarium culmorum: infection process, mechanisms of mycotoxin production and their role in pathogenesis in wheat. Crop Protection, 26:877–885. https://doi.org/10.1016/j.cropro.2006.09.003
  • Wakelin, S., Warren, R., Harvey, P., & Ryder, M. (2004). Phosphate solubilization by Penicillium spp. closely associated with wheat roots. Biology and Fertility of Soils, 40, 36–43. https://doi.org/10.1007/s00374-004-0750-6
  • Wallwork, H., Butt, M., Cheong, J. P. E., & Williams, K. J. (2004). Resistance to crown rot in wheat identified through an improved method for screening adult plants. Australian Plant Pathology Society, 33, 1–7. https://doi.org/10.1071/AP03073
  • Wang, H., Hwang, S. F., Eudes, F., Chang, K. F., Howard, R. J., & Turnbull, G. D. (2006). Trichothecenes and aggressiveness of Fusarium graminearum causing seedling blight and root rot in cereals. Plant Pathology, 55:224–230. https://doi.org/10.1111/j.1365-3059.2006.01339.x
  • Watanabe, I., Barraquıo, W. L., & Guzman, M. R. (1979). Nitrogen-fixing (acetylene reduction) activity and population of aerobic heterotrophic nitrogen- fixing bacteria associated with wetland rice. Applied and Environmental Microbiology, 37, 813–815. https://doi.org/10.1128/aem.37.5.813-819.1979
  • Wildermuth, G. B., & McNamara, R. B. (1994). Testing Wheat Seedlings For Resistance to Crown Rot Caused by Fusarium graminearum Group 1. Plant Disease, 78:949–953. https://doi.org/10.1094/PD-78-0949
  • Xu, F., Yang, G., Wang, J., Song, Y., Liu, L., Zhao, K., Li, Y., & Han, Z. (2018). Spatial distribution of root and crown rot fungi associated with winter wheat in the North China Plain and its relationship with climate variables. Frontiers in Microbiology, 9, 1054. https://doi.org/10.3389/fmicb.2018.01054
  • Zvyagintsev, D. G. (1991). Methods for Soil Microbiology and Biochemistry. Moscow State University, Moscow. (303 p.) (In Russian).
Toplam 61 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Fitopatoloji
Bölüm Bitki Koruma
Yazarlar

İnci Güler Güney 0000-0002-2544-8712

Sibel Derviş 0000-0002-4917-3813

Göksel Özer 0000-0002-3385-2520

Hüsnü Aktaş 0000-0001-6943-2109

Mehmet Ata Keske 0000-0001-9219-927X

Proje Numarası MAÜ.BAP.20.KMY.018
Erken Görünüm Tarihi 26 Nisan 2024
Yayımlanma Tarihi 29 Nisan 2024
Gönderilme Tarihi 6 Kasım 2023
Kabul Tarihi 8 Ocak 2024
Yayımlandığı Sayı Yıl 2024 Cilt: 10 Sayı: 1

Kaynak Göster

APA Güler Güney, İ., Derviş, S., Özer, G., Aktaş, H., vd. (2024). Determination of Antagonistic Activities of Endophytic Bacteria Isolated from Different Wheat Genotypes Against Fusarium culmorum. Uluslararası Tarım Ve Yaban Hayatı Bilimleri Dergisi, 10(1), 96-116. https://doi.org/10.24180/ijaws.1386741
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