Araştırma Makalesi
BibTex RIS Kaynak Göster

GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) ANALYSIS OF CONSOLIDA THIRKEANA EXTRACT

Yıl 2022, Cilt: 46 Sayı: 2, 444 - 449, 29.05.2022

Kenan Can Tok Şeyda Yayla

https://doi.org/10.33483/jfpau.1102380
Cited By: 2

Öz

Objective: Consolida thirkeana (Boiss.) Bornm. (Ranunculaceae) is considered endemic to Turkey and identified by pale lilac flowers, sessile follicles, and laciniae linear leaves. The genus Consolida can often be confused morphologically with Delphinium, in this respect, members of the genus Consolida need chemotaxonomic interest. In this study, the phytochemical content of C. thirkeana was clarified by GC/MS analysis.
Material and Method: The plant material was collected from Ayaş (Ankara/Turkey). A voucher specimen was deposited in the Ankara University Faculty of Pharmacy Herbarium. The aerial part of the plant was powdered in a grinder. The powdered plant parts were macerated with methanol. The GC-MS analysis of extracts was performed using an Agilent 6890 gas chromatograph equipped with an Agilent 5973N quadrupole mass spectrometer (Agilent, USA). Mass Hunter software (Qualitative Analysis B.07.00) and the NIST Mass Spectral Library (2014) were used for determining and identifying compounds.
Result and Discussion: The analysis results of this study showed the presence of 15 compounds in C. thirkeana methanol extract. The extract contains 0.65% Glycoside, 0.68% Fatty acid, 0.91% Acylglycerol, 2.41% Carboxylic acid, 38.5% Sugar, 56.86% Sugar alcohol.

Anahtar Kelimeler

Consolida thirkeana endemic GC/MS herba phytochemistry

Teşekkür

The authors thank Dr. M. Mesud HÜRKUL for the collection of plant materials.

Kaynakça

  • Brummitt, R.K. (1992). Vascular plant families and genera. Richmond, UK: Royal Botanic Gardens, Kew.
  • Heywood, V.H., Brummitt, R.K., Culham, A., Seberg, O. (2007). Flowering plant families of the world. Ontario: Firefly Books.
  • Davis, P.H. (1965). Flora of Turkey and the East Aegean Islands, (Vol. 1, pp.119-134). Edinburgh, UK: Edinburgh University Press.
  • Hürkul, M.M. (2021). Leaf, stem and root anatomy of Consolida thirkeana (Boiss.) Bornm. (Ranunculaceae). Journal of research in pharmacy, 25(4), 415-419. [CrossRef]
  • Gümüşok, S., Hürkul, M.M. (2021). Anatomy of Consolida orientalis (Gay) Schröd. (Ranunculaceae): Root, stem and leaf. İstanbul Journal of Pharmacy, 51(3), 3-4. [CrossRef]
  • POWO. (2022). Plants of the World Online. Facilitated by the Royal Botanic Gardens, Kew. http://www.plantsoftheworldonline.org Accessed: 10.04.2022.
  • Evans, W. (2002). Trease and Evans pharmacognosy (15th ed.). WB Saunders.
  • Metcalfe, C.R., Chalk, L. (1965). Anatomy of dicotyledones. (Vol. 2). Oxford, UK: Clarendon Press.
  • Hao, D.C. (2018). Ranunculales medicinal plants: Biodiversity, chemodiversity and pharmacotherapy. London, UK: Academic Press.
  • Yin, T., Cai, L., Ding, Z. (2020). A systematic review on the chemical constituents of the genus Consolida (Ranunculaceae) and their biological activities. RSC Advances, 10(58), 35072-35089. [CrossRef]
  • Güner, A., Aslan, S., Ekim, T., Vural, M., Babaç, M. T. (2012). Türkiye Bitkileri Listesi (Damarlı Bitkiler). Nezahat Gökyigit Botanik Bahçesi Yayınları, Flora Dizisi I.
  • Kıymacı, M.E., Tok, K.C., Hürkul, M.M. (2022). A study on phytochemical analysis and antibacterial activity of Quercus macranthera subsp. syspirensis (K. Koch) Menitsky branch and leaf extracts. Journal of Faculty of Pharmacy of Ankara University, 46(1), 160-169. [CrossRef]
  • Blau, K., Halket, J.M. (1993). Handbook of derivatives for chromatography. Wiley.
  • Awuchi, C.G., Echeta, K.C. (2019). Current developments in sugar alcohols: Chemistry, nutrition, and health concerns of sorbitol, xylitol, glycerol, arabitol, inositol, maltitol, and lactitol. International Journal of Advanced Academic Research, 5, 1-33.
  • Saraiva, A., Carrascosa, C., Raheem, D., Ramos, F., Raposo, A. (2020). Natural sweeteners: The relevance of food naturalness for consumers, food security aspects, sustainability and health impacts. International Journal of Environmental Research and Public Health, 17(17), 6285. [CrossRef]
  • Kroger, M., Meister, K., Kava, R. (2006). Low‐calorie sweeteners and other sugar substitutes: a review of the safety issues. Comprehensive reviews in food science and food safety, 5(2), 35-47. [CrossRef]
  • Ruiz-Ojeda, F.J., Plaza-Díaz, J., Sáez-Lara, M.J., Gil, A. (2019). Effects of sweeteners on the gut microbiota: a review of experimental studies and clinical trials. Advances in Nutrition, 10, 31-48. [CrossRef]
  • Mericli, A.H., Yazici, S., Eroglu-Ozkan, E., Sen, B., Kurtoglu, S., Ozcelik, H., Mericli, F. (2012). Norditerpenoid alkaloids from Consolida thirkeana and Consolida sulphurea. Chemistry of Natural Compounds, 48(3), 525-526.

CONSOLIDA THIRKEANA EKSTRESİNİN GAZ KROMATOGRAFİSİ-KÜTLE SPEKTROMETRESİ (GC-MS) ANALİZİ

Yıl 2022, Cilt: 46 Sayı: 2, 444 - 449, 29.05.2022

Kenan Can Tok Şeyda Yayla

https://doi.org/10.33483/jfpau.1102380
Cited By: 2

Öz

Amaç: Consolida thirkeana (Boiss.) Bornm. (Ranunculaceae) Türkiye için endemik olarak kabul edilir ve soluk leylak çiçekleri, sapsız foliküller ve linear lasiniat yaprakları ile tanımlanır. Consolida cinsi genellikle morfolojik olarak Delphinium ile karıştırılabilir, bu bakımdan Consolida cinsinin üyelerinin kemotaksonomik olarak araştırılmasına ihtiyaç duyulur. Bu çalışmada, C. thirkeana'nın fitokimyasal içeriği GC/MS analizi ile aydınlatılmıştır.
Gereç ve Yöntem: Bitki materyali Ayaş'tan (Ankara/Türkiye) toplanmıştır. Bir herbaryum örneği Ankara Üniversitesi Eczacılık Fakültesi Herbaryumu'na kaydedilmiştir. Bitkinin herbası bir öğütücüde toz haline getirildi. Toz haline getirilmiş bitki parçaları metanol kullanılarak maserasyon yöntemi ile ekstre edildi. Ekstrelerin GC-MS analizi, bir Agilent 5973N dört kutuplu kütle spektrometresi (Agilent, ABD) ile donatılmış bir Agilent 6890 gaz kromatografı kullanılarak yapıldı. Bileşiklerin belirlenmesi ve tanımlanması için Mass Hunter yazılımı (Qualitative Analysis B.07.00) ve NIST Mass Spectral Library (2014) kullanıldı.
Sonuç ve Tartışma: Bu çalışmanın analiz sonuçları, C. thirkeana metanol ekstresinde 15 bileşiğin varlığını göstermiştir. Ekstre %0.65 Glikozit, %0.68 Yağ asidi, %0.91 Açilgliserol, %2.41 Karboksilik asit, %38.5 Şeker, %56.86 Şeker alkolü içerir.

Anahtar Kelimeler

Consolida thirkeana endemik fitokimya GC/MS herba

Kaynakça

  • Brummitt, R.K. (1992). Vascular plant families and genera. Richmond, UK: Royal Botanic Gardens, Kew.
  • Heywood, V.H., Brummitt, R.K., Culham, A., Seberg, O. (2007). Flowering plant families of the world. Ontario: Firefly Books.
  • Davis, P.H. (1965). Flora of Turkey and the East Aegean Islands, (Vol. 1, pp.119-134). Edinburgh, UK: Edinburgh University Press.
  • Hürkul, M.M. (2021). Leaf, stem and root anatomy of Consolida thirkeana (Boiss.) Bornm. (Ranunculaceae). Journal of research in pharmacy, 25(4), 415-419. [CrossRef]
  • Gümüşok, S., Hürkul, M.M. (2021). Anatomy of Consolida orientalis (Gay) Schröd. (Ranunculaceae): Root, stem and leaf. İstanbul Journal of Pharmacy, 51(3), 3-4. [CrossRef]
  • POWO. (2022). Plants of the World Online. Facilitated by the Royal Botanic Gardens, Kew. http://www.plantsoftheworldonline.org Accessed: 10.04.2022.
  • Evans, W. (2002). Trease and Evans pharmacognosy (15th ed.). WB Saunders.
  • Metcalfe, C.R., Chalk, L. (1965). Anatomy of dicotyledones. (Vol. 2). Oxford, UK: Clarendon Press.
  • Hao, D.C. (2018). Ranunculales medicinal plants: Biodiversity, chemodiversity and pharmacotherapy. London, UK: Academic Press.
  • Yin, T., Cai, L., Ding, Z. (2020). A systematic review on the chemical constituents of the genus Consolida (Ranunculaceae) and their biological activities. RSC Advances, 10(58), 35072-35089. [CrossRef]
  • Güner, A., Aslan, S., Ekim, T., Vural, M., Babaç, M. T. (2012). Türkiye Bitkileri Listesi (Damarlı Bitkiler). Nezahat Gökyigit Botanik Bahçesi Yayınları, Flora Dizisi I.
  • Kıymacı, M.E., Tok, K.C., Hürkul, M.M. (2022). A study on phytochemical analysis and antibacterial activity of Quercus macranthera subsp. syspirensis (K. Koch) Menitsky branch and leaf extracts. Journal of Faculty of Pharmacy of Ankara University, 46(1), 160-169. [CrossRef]
  • Blau, K., Halket, J.M. (1993). Handbook of derivatives for chromatography. Wiley.
  • Awuchi, C.G., Echeta, K.C. (2019). Current developments in sugar alcohols: Chemistry, nutrition, and health concerns of sorbitol, xylitol, glycerol, arabitol, inositol, maltitol, and lactitol. International Journal of Advanced Academic Research, 5, 1-33.
  • Saraiva, A., Carrascosa, C., Raheem, D., Ramos, F., Raposo, A. (2020). Natural sweeteners: The relevance of food naturalness for consumers, food security aspects, sustainability and health impacts. International Journal of Environmental Research and Public Health, 17(17), 6285. [CrossRef]
  • Kroger, M., Meister, K., Kava, R. (2006). Low‐calorie sweeteners and other sugar substitutes: a review of the safety issues. Comprehensive reviews in food science and food safety, 5(2), 35-47. [CrossRef]
  • Ruiz-Ojeda, F.J., Plaza-Díaz, J., Sáez-Lara, M.J., Gil, A. (2019). Effects of sweeteners on the gut microbiota: a review of experimental studies and clinical trials. Advances in Nutrition, 10, 31-48. [CrossRef]
  • Mericli, A.H., Yazici, S., Eroglu-Ozkan, E., Sen, B., Kurtoglu, S., Ozcelik, H., Mericli, F. (2012). Norditerpenoid alkaloids from Consolida thirkeana and Consolida sulphurea. Chemistry of Natural Compounds, 48(3), 525-526.
Toplam 18 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Eczacılık ve İlaç Bilimleri
Bölüm Araştırma Makalesi
Yazarlar

Kenan Can Tok 0000-0002-9353-8867

Şeyda Yayla 0000-0002-3678-6506

Yayımlanma Tarihi 29 Mayıs 2022
Gönderilme Tarihi 12 Nisan 2022
Kabul Tarihi 21 Nisan 2022
Yayımlandığı Sayı Yıl 2022 Cilt: 46 Sayı: 2

Kaynak Göster

APA Tok, K. C., & Yayla, Ş. (2022). GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) ANALYSIS OF CONSOLIDA THIRKEANA EXTRACT. Journal of Faculty of Pharmacy of Ankara University, 46(2), 444-449. https://doi.org/10.33483/jfpau.1102380
AMA Tok KC, Yayla Ş. GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) ANALYSIS OF CONSOLIDA THIRKEANA EXTRACT. Ankara Ecz. Fak. Derg. Mayıs 2022;46(2):444-449. doi:10.33483/jfpau.1102380
Chicago Tok, Kenan Can, ve Şeyda Yayla. “GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) ANALYSIS OF CONSOLIDA THIRKEANA EXTRACT”. Journal of Faculty of Pharmacy of Ankara University 46, sy. 2 (Mayıs 2022): 444-49. https://doi.org/10.33483/jfpau.1102380.
EndNote Tok KC, Yayla Ş (01 Mayıs 2022) GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) ANALYSIS OF CONSOLIDA THIRKEANA EXTRACT. Journal of Faculty of Pharmacy of Ankara University 46 2 444–449.
IEEE K. C. Tok ve Ş. Yayla, “GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) ANALYSIS OF CONSOLIDA THIRKEANA EXTRACT”, Ankara Ecz. Fak. Derg., c. 46, sy. 2, ss. 444–449, 2022, doi: 10.33483/jfpau.1102380.
ISNAD Tok, Kenan Can - Yayla, Şeyda. “GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) ANALYSIS OF CONSOLIDA THIRKEANA EXTRACT”. Journal of Faculty of Pharmacy of Ankara University 46/2 (Mayıs 2022), 444-449. https://doi.org/10.33483/jfpau.1102380.
JAMA Tok KC, Yayla Ş. GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) ANALYSIS OF CONSOLIDA THIRKEANA EXTRACT. Ankara Ecz. Fak. Derg. 2022;46:444–449.
MLA Tok, Kenan Can ve Şeyda Yayla. “GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) ANALYSIS OF CONSOLIDA THIRKEANA EXTRACT”. Journal of Faculty of Pharmacy of Ankara University, c. 46, sy. 2, 2022, ss. 444-9, doi:10.33483/jfpau.1102380.
Vancouver Tok KC, Yayla Ş. GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) ANALYSIS OF CONSOLIDA THIRKEANA EXTRACT. Ankara Ecz. Fak. Derg. 2022;46(2):444-9.

Cited By

Quantification of Some Phenolic Compounds in Consolida thirkeana (Boiss.) Bornm. by HPLC and Validation of Method
Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi
https://doi.org/10.18016/ksutarimdoga.vi.1210096
SILENE RUSCIFOLIA METANOLİK HERBA EKSTRESİNDEKİ FİTOKİMYASALLARIN LC-QTOF/MS VE GC/MS İLE İNCELENMESİ
Ankara Universitesi Eczacilik Fakultesi Dergisi
https://doi.org/10.33483/jfpau.1133615
 Kapak Resmi İndir

Kapsam ve Amaç

Ankara Üniversitesi Eczacılık Fakültesi Dergisi, açık erişim, hakemli bir dergi olup Türkçe veya İngilizce olarak farmasötik bilimler alanındaki önemli gelişmeleri içeren orijinal araştırmalar, derlemeler ve kısa bildiriler için uluslararası bir yayım ortamıdır. Bilimsel toplantılarda sunulan bildiriler supleman özel sayısı olarak dergide yayımlanabilir. Ayrıca, tüm farmasötik alandaki gelecek ve önceki ulusal ve uluslararası bilimsel toplantılar ile sosyal aktiviteleri içerir.