CHEMICAL COMPOSITION AND TOXIC EFFECTS OF ESSENTIAL OIL OF Origanum rotundifolium BOISS. ON ZEA MAYS SEEDLINGS
Öz
This study was conducted to investigate essential oil based genetical and physiological changes on maize seedling under laboratory conditions. The aerial parts of O. rotundifolium BOISS. Were powdered, and EO obtained by hydro-distillation. For the best results, the content applied to Gas Chromatography/ Mass Spectrometry (GC-MS), and the main constituents have been determined as Carvacrol (%43.62) and Thymol (%40.86). The liquid phase of the oil applied to maize seeds at four concentrations. Randomly Amplified Polymorphic DNA method was used to observe possible DNA alterations. With the total soluble protein level and SDS Page analysis, the physiological effects of EO were determined. The oils showed phytotoxic effects, but not in a dose-dependent manner. Soluble protein level and SDS Page analysis did not show any significant change but genomic template stability value was decreased with the increasing of EO dose.
Anahtar Kelimeler
Kaynakça
- Dikbas, N., R. Kotan, F. Dadasoglu, K. Karagöz, and R. Çakmakcý, Correlation between major constituents and antibacterial activities of some plant essential oils against some pathogenic bacteria, Turkish Journal of Science & Technology, 4 (1), 57-64, 2009.
- Goze, I., A. Alim, A.S. Tepe, M. Sokmen, K. Sevgi, and B. Tepe, Screening of the antioxidant activity of essential oil and various extracts of Origanum rotundifolium Boiss. from Turkey, Journal of Medicinal Plants Research, 3 (4), 246-254, 2009.
- Khanh, T.D., T.D. Xuan, and I.M. Chung, Rice allelopathy and the possibility for weed management, Annals of Applied Biology, 151 (3), 325-339, 2007. 4. Waller, G., Introduction-reality and future of allelopathy, Allelopathy: Chemistry and Mode of Action of Allelochemicals (eds. Macias FA, Galindo JCG, Molinilla HMG, Cutler HG), 1-12, 2004.
- Waller, G., Introduction-reality and future of allelopathy, Allelopathy: Chemistry and Mode of Action of Allelochemicals (eds. Macias FA, Galindo JCG, Molinilla HMG, Cutler HG), 1-12, 2004.
- Duke, S. and A. Oliva, Mode of action of phytotoxic terpenoids CRC Press, Boca Raton, FL, 2004.
- Chou, C.H., Introduction to allelopathy, Allelopathy, 1-9, 2006.
- Vasilakoglu, I., K. Dhima, E. Wogiatzi, I. Eleftherohorinos, and A. Lithourgidis, Herbicidal potential of essential oils of oregano or marjoram (Origanum spp.) and basil (Ocimum basilicum) on Echinochloa crus-galli (L.) P. Beauv. An Chenopodium album L. weeds, Allelopathy Journal, 20 (2), 297-305, 2007.
- Cohn, B.A., Developmental and environmental origins of breast cancer: DDT as a case study, Reproductive Toxicology, 31 (3), 302-311, 2011.
- Weston, D.P., J. You, and M.J. Lydy, Distribution and toxicity of sedimentassociated pesticides in agriculture-dominated water bodies of California’s Central Valley, Environmental Science & Technology, 38 (10), 2752-2759, 2004.
- Li, G. and C.F. Quiros, Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica, Theoretical and Applied Genetics, 103 (2-3), 455- 461, 2001.
- Agar, G., S. Bozari, A. Adiguzel, O. Baris, M. Gulluce, M. Sengul, and F. Sahin, Phenotypic and genetic variation among Astragalus species from Turkey, Romanian Biotechnological Letters, 14 (2), 4267-4274, 2009.
- Bradford, M.M., Rapid snd Sensitive Method for Quantitation of Microgram Quantities of Protein Utilizing Principle of Protein-Dye Binding, Analytical Biochemistry, 72 (1-2), 248-254, 1976.
- Shultz, R.W., S.B. Settlage, L. Hanley-Bowdoin, and W.F. Thompson, A trichloroacetic acid-acetone method greatly reduces infrared autofluorescence of protein extracts from plant tissue, Plant Molecular Biology Reporter, 23 (4), 405-409, 2005.
- Laemmli, U.K., Cleavage of Structural Proteins during Assembly of Head of Bacteriophage-T4, Nature, 227 (5259), 680-&, 1970.
- Franz, C. and J. Novak, Sources of Essential Oils, Handbook of Essential Oils: Science, Technology, and Applications, 39, 2009.
- Harzallah, H.J., B. Kouidhi, G. Flamini, A. Bakhrouf, and T. Mahjoub, Chemical composition, antimicrobial potential against cariogenic bacteria and cytotoxic activity of Tunisian Nigella sativa essential oil and thymoquinone, Food Chemistry, 129 (4), 1469-1474, 2011.
- Basile, A., R.C. Cobianchi, D. Rigano, F. Senatore, M. Bruno, S. Rosselli, B. Conte, and S. Sorbo, Potential allelopathic activity of Sideritis italica (Miller) Greuter et Burdet essential oil, Plant Biosystems, 145 (1), 241-247, 2011.
- Zahed, N., K. Hosni, N. Ben Brahim, M. Kallel, and H. Sebei, Allelopathic effect of Schinus molle essential oils on wheat germination, Acta Physiologiae Plantarum, 32 (6), 1221-1227, 2010.
- Dadasoglu, F., T. Aydin, R. Kotan, A. Cakir, H. Ozer, S. Kordali, R. Cakmakci, N. Dikbas, and E. Mete, Antibacterial Activities Of Extracts And Essential Oils Of Three Origanum Species Against Plant Pathogenic Bacteria And Their Potential Use As Seed Disinfectants, Journal of Plant Pathology, 93 (2), 271- 282, 2011.
- Dikbas, N., E. Bagci, R. Kotan, R. Cakmakci, H. Ozer, E. Mete, and G. Erdogan, Comparative antibacterial activities and chemical composition of some plants’ oils against Salmonella enteritidis, Research on Crops, 11 (1), 118-124, 2010.
- Cetin, B., S. Cakmakci, and R. Cakmakci, The investigation of antimicrobial activity of thyme and oregano essential oils, Turkish Journal of Agriculture and Forestry, 35 (2), 145-154, 2011.
- Chen, J.Z., J.Y. Ye, H.Y. Zhang, X.J. Jiang, Y.X. Zhang, and Z.L. Liu, Freshwater toxic cyanobacteria induced DNA damage in apple (Malus pumila), rape (Brassica napus) and rice (Oryza sativa), Journal of Hazardous Materials, 190 (1-3), 240-244, 2011.
- Theodorakis, C.W. and J.W. Bickham, Molecular characterization of contaminant-indicative RAPD markers, Ecotoxicology, 13 (4), 303-309, 2004.
- Carroll, L., WHAT IS ALLELOPATHY?, The History of Allelopathy, 1, 2007.
- Bozari, S., G. Agar, O. Aksakal, F.A. Erturk, and D. Yanmis, Determination of chemical composition and genotoxic effects of essential oil obtained from Nepeta nuda on Zea mays seedlings, Toxicology and Industrial Health, 2012.
- Hong, L.Z., Radix linderae essential oil improving the immunity activities and preventing the occurrence of decubitus in aged people, Journal of Medicinal Plants Research, 5 (16), 3733-3738, 2011.
- Zhang, D.L., H.P. Hu, Q.X. Rao, and Z.H. Zhao, Synergistic Effects and Physiological Responses of Selected Bacterial Isolates from Animal Feed to Four Natural Antimicrobials and Two Antibiotics, Foodborne Pathogens And Disease, 8 (10), 1055-1062, 2011.
Origanum rotundifolium BOISS.’İN UÇUCU YAĞLARININ KİMYASAL BİLEŞİMİ VE Zea mays TOHUMLARININ ÇİMLENMESİ ÜZERİNE TOKSİK ETKİLERİ
Öz
Bu çalışma; uçucu yağların laboratuvar şartlarında mısır fidelerinde neden olduğu genetik ve fizyolojik hasarları belirlemek amacıyla yapıldı. O. rotundifolium BOISS. bitkisinin toprak üstü organları toz haline getirilip hidrodistilasyon yöntemiyle uçucu yağları elde edildi. Doğru sonuç elde etmek için uçucu yağların içeriği GC-MS yöntemiyle belirlenip temel bileşenleri belirlenip en fazla bulunan içeriklerin %43.62 oranıyla Carvacrol ve %40.86 oranıyla Thymol olduğu tespit edildi. Sıvı haldeki toplam içerik mısır tohumlarına dört farklı dozda uygulandı. RAPD analizleriyle olası genetiksel değişimler belirlendi. Toplam çözünür protein miktarı ve SDS Page profilleriyle ise fizyolojik değişimler tespit edildi. Uçucu yağların dozdan bağımsız fitotoksik etki yarattığı gözlendi. Genomik stabilitenin artan uçucu yağ konsantrasyonuna bağlı olarak düştüğü ancak bu değişimin çözünür protein miktarı ile protein profillerini etkilemediği belirlendi.
Anahtar Kelimeler
Kaynakça
- Dikbas, N., R. Kotan, F. Dadasoglu, K. Karagöz, and R. Çakmakcý, Correlation between major constituents and antibacterial activities of some plant essential oils against some pathogenic bacteria, Turkish Journal of Science & Technology, 4 (1), 57-64, 2009.
- Goze, I., A. Alim, A.S. Tepe, M. Sokmen, K. Sevgi, and B. Tepe, Screening of the antioxidant activity of essential oil and various extracts of Origanum rotundifolium Boiss. from Turkey, Journal of Medicinal Plants Research, 3 (4), 246-254, 2009.
- Khanh, T.D., T.D. Xuan, and I.M. Chung, Rice allelopathy and the possibility for weed management, Annals of Applied Biology, 151 (3), 325-339, 2007. 4. Waller, G., Introduction-reality and future of allelopathy, Allelopathy: Chemistry and Mode of Action of Allelochemicals (eds. Macias FA, Galindo JCG, Molinilla HMG, Cutler HG), 1-12, 2004.
- Waller, G., Introduction-reality and future of allelopathy, Allelopathy: Chemistry and Mode of Action of Allelochemicals (eds. Macias FA, Galindo JCG, Molinilla HMG, Cutler HG), 1-12, 2004.
- Duke, S. and A. Oliva, Mode of action of phytotoxic terpenoids CRC Press, Boca Raton, FL, 2004.
- Chou, C.H., Introduction to allelopathy, Allelopathy, 1-9, 2006.
- Vasilakoglu, I., K. Dhima, E. Wogiatzi, I. Eleftherohorinos, and A. Lithourgidis, Herbicidal potential of essential oils of oregano or marjoram (Origanum spp.) and basil (Ocimum basilicum) on Echinochloa crus-galli (L.) P. Beauv. An Chenopodium album L. weeds, Allelopathy Journal, 20 (2), 297-305, 2007.
- Cohn, B.A., Developmental and environmental origins of breast cancer: DDT as a case study, Reproductive Toxicology, 31 (3), 302-311, 2011.
- Weston, D.P., J. You, and M.J. Lydy, Distribution and toxicity of sedimentassociated pesticides in agriculture-dominated water bodies of California’s Central Valley, Environmental Science & Technology, 38 (10), 2752-2759, 2004.
- Li, G. and C.F. Quiros, Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica, Theoretical and Applied Genetics, 103 (2-3), 455- 461, 2001.
- Agar, G., S. Bozari, A. Adiguzel, O. Baris, M. Gulluce, M. Sengul, and F. Sahin, Phenotypic and genetic variation among Astragalus species from Turkey, Romanian Biotechnological Letters, 14 (2), 4267-4274, 2009.
- Bradford, M.M., Rapid snd Sensitive Method for Quantitation of Microgram Quantities of Protein Utilizing Principle of Protein-Dye Binding, Analytical Biochemistry, 72 (1-2), 248-254, 1976.
- Shultz, R.W., S.B. Settlage, L. Hanley-Bowdoin, and W.F. Thompson, A trichloroacetic acid-acetone method greatly reduces infrared autofluorescence of protein extracts from plant tissue, Plant Molecular Biology Reporter, 23 (4), 405-409, 2005.
- Laemmli, U.K., Cleavage of Structural Proteins during Assembly of Head of Bacteriophage-T4, Nature, 227 (5259), 680-&, 1970.
- Franz, C. and J. Novak, Sources of Essential Oils, Handbook of Essential Oils: Science, Technology, and Applications, 39, 2009.
- Harzallah, H.J., B. Kouidhi, G. Flamini, A. Bakhrouf, and T. Mahjoub, Chemical composition, antimicrobial potential against cariogenic bacteria and cytotoxic activity of Tunisian Nigella sativa essential oil and thymoquinone, Food Chemistry, 129 (4), 1469-1474, 2011.
- Basile, A., R.C. Cobianchi, D. Rigano, F. Senatore, M. Bruno, S. Rosselli, B. Conte, and S. Sorbo, Potential allelopathic activity of Sideritis italica (Miller) Greuter et Burdet essential oil, Plant Biosystems, 145 (1), 241-247, 2011.
- Zahed, N., K. Hosni, N. Ben Brahim, M. Kallel, and H. Sebei, Allelopathic effect of Schinus molle essential oils on wheat germination, Acta Physiologiae Plantarum, 32 (6), 1221-1227, 2010.
- Dadasoglu, F., T. Aydin, R. Kotan, A. Cakir, H. Ozer, S. Kordali, R. Cakmakci, N. Dikbas, and E. Mete, Antibacterial Activities Of Extracts And Essential Oils Of Three Origanum Species Against Plant Pathogenic Bacteria And Their Potential Use As Seed Disinfectants, Journal of Plant Pathology, 93 (2), 271- 282, 2011.
- Dikbas, N., E. Bagci, R. Kotan, R. Cakmakci, H. Ozer, E. Mete, and G. Erdogan, Comparative antibacterial activities and chemical composition of some plants’ oils against Salmonella enteritidis, Research on Crops, 11 (1), 118-124, 2010.
- Cetin, B., S. Cakmakci, and R. Cakmakci, The investigation of antimicrobial activity of thyme and oregano essential oils, Turkish Journal of Agriculture and Forestry, 35 (2), 145-154, 2011.
- Chen, J.Z., J.Y. Ye, H.Y. Zhang, X.J. Jiang, Y.X. Zhang, and Z.L. Liu, Freshwater toxic cyanobacteria induced DNA damage in apple (Malus pumila), rape (Brassica napus) and rice (Oryza sativa), Journal of Hazardous Materials, 190 (1-3), 240-244, 2011.
- Theodorakis, C.W. and J.W. Bickham, Molecular characterization of contaminant-indicative RAPD markers, Ecotoxicology, 13 (4), 303-309, 2004.
- Carroll, L., WHAT IS ALLELOPATHY?, The History of Allelopathy, 1, 2007.
- Bozari, S., G. Agar, O. Aksakal, F.A. Erturk, and D. Yanmis, Determination of chemical composition and genotoxic effects of essential oil obtained from Nepeta nuda on Zea mays seedlings, Toxicology and Industrial Health, 2012.
- Hong, L.Z., Radix linderae essential oil improving the immunity activities and preventing the occurrence of decubitus in aged people, Journal of Medicinal Plants Research, 5 (16), 3733-3738, 2011.
- Zhang, D.L., H.P. Hu, Q.X. Rao, and Z.H. Zhao, Synergistic Effects and Physiological Responses of Selected Bacterial Isolates from Animal Feed to Four Natural Antimicrobials and Two Antibiotics, Foodborne Pathogens And Disease, 8 (10), 1055-1062, 2011.
Toplam 27 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Haziran 2013 |
| Yayımlandığı Sayı | Yıl 2013 Cilt: 1 Sayı: 1 |